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Cloning and Expression
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LongAmpHot StartTaq2X Master Mix
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LongAmpHot StartTaq2X Master Mix
LongAmpHot StartTaq2X Master Mix from New England Biolabs
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Citations:
(10)
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LongAmpHot StartTaq2X Master Mix
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Company Info
Citations
Reviews
Product Specs
Item
LongAmpHot StartTaq2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0533L
Quantity
500 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (10)
(1)
Competition between skin antimicrobial peptides and commensal bacteria in type 2 inflammation enables survival of S. aureus
Cell Reports
May 30, 2023
Teruaki Nakatsuji, Samantha L. Brinton, Kellen J. Cavagnero, Alan M. O’Neill, Yang Chen, Tatsuya Dokoshi, Anna M. Butcher, Olive C. Osuoji, Faiza Shafiq, Josh L. Espinoza,
et al.
(GLL-33),"Genemed Synthesis, Inc.",Custom Peptide LongAmp Hot Start
Taq 2X Master Mix
,
New England BioLabs
,Cat#:
M0533S
Agencourt AMPure XP beads,
Beckman Coulter
,Cat#:
A63881
,, Critical commercial
assays
,, ,, PureLink
(2)
Capture-seq protocol and TE-reX pipeline guidelines for detection of recombination of repeat elements in short- and long-DNA reads libraries
STAR Protocols
March 1, 2023
Giovanni Pascarella, Letizia Straniero, Martin Frith, Piero Carninci
Index Primer Pairs),
New England Biolabs
,
E6440S
LongAmp Hot Start
Taq 2X Master Mix
,
New England Biolabs
,
M0533L
High
Sensitivity
DNA kit,
Agilent
,
5067-4626
RNA 6000 Nano Kit,
Agilent
,
5067-1511
MEGAscript T7 Transcription Kit
(3)
A wide foodomics approach coupled with metagenomics elucidates the environmental signature of potatoes
iScience
January 20, 2023
Anastasia Boutsika, Michail Michailidis, Maria Ganopoulou, Athanasios Dalakouras, Christina Skodra, Aliki Xanthopoulou, George Stamatakis, Martina Samiotaki, Georgia Tanou, Theodoros Moysiadis,
et al.
Applied Biosystems® QuantStudio® 5 Real-Time PCR System
(
Thermo Fischer Scientific
, Waltham, MA, USA), using a LongAmp Hot Start
Taq 2x Master Mix
(
M0533S
,
New England Biolabs
), and 16S barcoded primers. The 16S Barcoding Kit 1-24 (SQK-16S024, Oxford Nanopore
(4)
INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing
Genome Biology
October 25, 2022
Dimitrije IvanÄić, Júlia Mir-Pedrol, Jessica Jaraba-Wallace, Núria Rafel, Avencia Sanchez-Mejias, Marc Güell
single-tail adapter/tag (STAT)-
PCR
[29] were performed with LongAmp Hot Start
Taq polymerase
(
NEB
,
M0533S
) using adaptor binding P5_1 and P5_2 primers and corresponding insert primer pairs (see Table S2). For
(5)
Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9
Nucleic Acids Research
April 22, 2022
Kyung W Yoo, Manish Kumar Yadav, Qianqian Song, Anthony Atala, Baisong Lu
region of 4862 bp was amplified by LongAmp® Hot Start Taq 2× Master Mix (
New England Biolabs
, catalog #
M0533
) with primers hCLCN5-F3 and hCLCN5-R3. DNA was submitted to the GCB
Sequencing
and Genomic Technologies
(6)
Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner
Redox biology
April 1, 2022
Shin J, Hong SG, Choi SY, Rath ME, Saredy J, Jovin DG, Sayoc J, Park HS, Eguchi S, Rizzo V,
et al
.
DNA from each sample were amplified with LongAmp Hot Start Tag 2X Master Mix polymerase Taq (
NEB
, #
M0533S
) for long amplicon (LA) and short amplicon (SA). The
PCR
products were quantified using PicoGreen dye
(7)
High-speed rail model reveals the gene tandem amplification mediated by short repeated sequence in eukaryote
Scientific Reports
February 10, 2022
Haidi Chen, Jingwen Xue, Zhenghou Zhang, Geyu Zhang, Xinyuan Xu, He Li, Ruxue Zhang, Najeeb Ullah, Lvxing Chen, Amanullah,
et al.
dried and finally resuspended in 100 µl sterile dH2O. Genomic DNA was amplified using a
long PCR
kit (
M0533S
,
NEB
)49. Then the products were collected for
sequencing
(PacBio Sequel II, CCS model). Gene synteny
(8)
Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons
Nature Communications
December 1, 2021
Karim Rahimi, Morten T. Venø, Daniel M. Dupont, Jørgen Kjems
circRNA. To do
RT-PCR
for microexon validation, LongAmp Hot Start
Taq 2X Master Mix
(
New England Biolabs
,
M0533L
) was used following the manufacturer’s instruction. Sequence of the primers provided in Supplementary
(9)
Generation of mouse conditional knockout alleles in one step using the
i
-GONAD method
Genome Research
January 1, 2021
Renjie Shang, Haifeng Zhang, Pengpeng Bi
with T7 or SP6 primers.
Long-range PCR
was performed using LongAmp Hot Start
Taq 2X Master Mix
(
NEB
M0533S
) to examine genomic DNA that was purified by a
Monarch Genomic DNA Purification kit
(
NEB
T3010). The
(10)
Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells
STAR protocols
June 19, 2020
Tran NT, Trombke J, Rajewsky K, Chu VT.
Kit (VioBright™ FITC),Miltenyi Biotec,Cat#130-123-124 LongAmp PCR Master Mix,
New England Biolabs
,Cat#
M0533L
KOD Hot Start DNA Polymerase
,
Millipore
,Cat#
71086-3
p.JET TOPO
cloning
Kit,Thermo Fischer,Cat#K1231
T7 endonuclease I
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LongAmpHot StartTaq2X Master Mix from New England Biolabs
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